uxaA [2019-07-31 10:36:16]

altronate hydrolase

Locus: BSU_12390

Isoelectric point: 5.78

Molecular weight: 54.75 kDa

Protein length: 497 aa

Gene length: 1494 bp

Function: hexuronate utilization

Product: altronate hydrolase

Essential: no

E.C.: 4.2.1.7

Synonyms: yjmJ

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of hexuronate
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 1,311,319 → 1,312,812

The protein

Catalyzed reaction/ biological activity

• D-altronate = 2-dehydro-3-deoxy-D-gluconate + H₂O (according to Swiss-Prot)

Protein family

• UxaA family (with YcbH, according to UniProt)

Expression and Regulation

Operons

• Genes: yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigE: sigma factor, PubMed, in SigE regulon

  Regulatory mechanism

  • ExuR: repression, in the absence of inducer, in ExuR regulon
  • CcpA: repression, PubMed PubMed, in CcpA regulon

  Regulation

  • induced by galacturonate (ExuR) PubMed

• Genes: uxaC-yjmB-yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ExuR: repression, in the absence of inducer, in ExuR regulon
  • CcpA: repression, PubMed PubMed, in CcpA regulon

  Regulation

  • induced by galacturonate (ExuR) PubMed

Biological materials

Mutant

• MGNA-A374 (yjmJ::erm), available at the NBRP B. subtilis, Japan
• BKE12390 (ΔuxaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTCAACGTACCTCTCCCCC, downstream forward: _UP4_TAATAAGAAAAGGCTATCCT
• BKK12390 (ΔuxaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTCAACGTACCTCTCCCCC, downstream forward: _UP4_TAATAAGAAAAGGCTATCCT

References

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